By Professor Saul B. Needleman (auth.)

Confusion now hath made his masterpiece Macbeth II iii seventy two Whence and what are these execrable form? Paradise misplaced Ib 1 681 Confusion worse confounded Paradise misplaced Ib 1 995 whilst the manuscript for the 1st a part of this e-book used to be proposed, it used to be expected that the dialogue of the whole box of protein se­ quencing can be lined in one quantity - from purification and characterization of the protein via fragmentation through chemical or enzymic skill and, ultimately, to reassembly of the pointed out person peptides into the reconstructed overall series. It quickly grew to become glaring that this may now not be attainable. whereas the cause used to be to limit the disclose of strategies in basic terms to that details enthusiastic about "hands on" rainy chemistry, it grew to become obvious thorough presentation will require, additionally, a dialogue of definite instrumental and extra theoretical ways now not incorporated within the first quantity. additionally, the complete knowing of the sector of protein sequencing has complicated considerably because the inception of this booklet. the aim of the 1st quantity was once to supply sensible details in enough aspect to allow the researcher to adopt the particular sequencing techniques in his personal laboratory.

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In another experi- 33 .. I. --------------Lys,s------------Hse44-Restl t Trypsin ~ Hse coupling II. ------------------------Hse44-Resin Fig. 14. Hypothetical strategy for sequencing the N-terminal BrCN fragment of acyl carrier protein. I the peptide is coupled and the N-terminal segment sequenced. II a sample of peptide is cleaved with trypsin and the C-terminal segment is coupled and sequenced ment, the cyanogen bromide-digested protein can be further digested with trypsin, and the resulting peptide mixture coupled again using the homoserine lactone method and sequenced.

The pellet or subpellets are stored frozen 46 at -70°. Enzyme preparations at this stage of purification have been employed successfully in the removal of peA residues from a large molecular weight protein without detecting any other proteolysis (21). The material ranges from 60-100-fold purified over the crude bacterial extract. Additional purification is not necessary if the enzyme is only to be used as a reagent in protein structure work. In fact, because the enzyme activity tends to become less stable with increasing purification, it is usually undesirable to carry the purification further.

4). However, the environment at the C-terminal carboxyl is unique in that azlactone formation is possible. Addition of the amino resin to the azlactone results in a peptide bound specifically at the C-terminus. Unfortunately the yields of peptide linkage by this and other versions of the carbodiimide attachment procedure are frequently very low and unpredictable. The most likely reason for the low yields is N-acylurea formation at the C-terminus, which competes with the desired reactions and inactivates the peptide.

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