By Zoe A. Shabarova, Alexey A. Bogdanov
Sequencing, cloning, transcription - those are yet a couple of key thoughts in the back of the present breathtaking advances in molecular biology and biochemistry. As those equipment continuosly diversify, biochemists desire a sound chemical figuring out to maintain the speed. Chemists starting operating within the molecular biology lab desire an advent to this box from their viewpoint. This ebook serves either: it describes lots of the identified chemical reactions of nucleosides, nucleotides, and nucleic acids in enough element to supply the specified history, and also, the elemental kin among series, constitution and performance of nucleic acids are presented.The first variation of this publication, which was once released in Russian, has instantly turn into a famous typical reference. This moment, completely revised and up to date version, now released in English, is probably going to accomplish an identical place within the foreign medical neighborhood.
Read or Download Advanced Organic Chemistry of Nucleic Acids PDF
Similar biochemistry books
Practical Biochemistry in overall healthiness and disorder presents a transparent and easy account of the biochemistry that's essential to comprehend the physiological features of tissues or organs necessary to the lifetime of humans. concentrating on the dynamic points of biochemistry and its software to the elemental services of the physique, the booklet bridges the space among biochemistry and scientific perform.
Explosive development within the variety of organic reagents on hand on the market to the study group has considerably advanced the method of discovering a specific chemical or finding an appropriate substitute for person who isn't any longer offered. development at the luck in their SourceBook of Enzymes, John and Dorothy Chong White have assembled an exhaustive catalog of over 26,000 commercially on hand proteins, peptides, and amino acids, all prepared alphabetically and by way of series for speedy entry, and every replete with technical information and seller info.
This ebook offers a complete survey of contemporary advancements and purposes of excessive functionality capillary electrophoresis within the box of protein and peptide research with a different specialise in the research of intact proteins. With useful element, the contents conceal varied modes of capillary electrophoresis (CE) priceless for protein and peptide research, CZE, CIEF, ACE, CGE, and forms of software similar to the standard keep an eye on of healing proteins and monoclonal antibodies, scientific analyses of chemokines in tissues, qualitative and quantitative research of vaccine proteins, and backbone of binding constants in complexes related to peptides or proteins.
Extra info for Advanced Organic Chemistry of Nucleic Acids
The amino group in guanosine does not seem to be modified by hydroxylamine and its 0-alkyl derivative. Only hydroxylamine reacts with uridine and only in an alkaline medium. This reaction leads to the rupture of the heterocycle. This is why it will not be considered here, although the reaction is extensively used for specific modification of nucleic acids at uracil units. Another group of nucleophilic reagents is represented by hydrazine and its derivatives. The reactions involving them are conducted in an aqueous medium, their mechanism being heavily dependent on the pH of the latter.
The most typical reaction in this category is amino group acylation (the acylation also involving the hydroxy groups of the carbohydrate moiety). Of the three major nucleosides containing amino groups in the heterocyclic nucleus, cytidine enters into acylation reactions most easily. For selective N-acetylation the reaction is carried out in an alcohol solution with boiling in the presence of an equimolar amount of acetic anhydride. The structure of the acetylation products has been established by U y IR and NMR spectroscopy.
The corresponding 5-amino nucleosides can be obtained by reduction of the 5-nitro derivatives. Heating of uridine together with formaldehyde in the presence of hydrochloric acid leads to its 5-hydroxymethylation. 0 0 I I R R R-ribose residue Under similar conditions, cytidine does not undergo hydroxymethylation (probably because of protonation of the amino group whose positive charge prevents electrophilic substitution). 5-Hydroxymethyluracil is easily formed when the base is hydroxymethylated in an alkaline medium.