By Louise M. Steele, Margaret M. Sedensky, Phil G. Morgan (auth.), Arpad Szallasi (eds.)
Chronic soreness is a posh phenomenon, which keeps to stay undertreated within the majority of affected sufferers hence representing an important unmet clinical desire, however the improvement of mobile, subcellular, and molecular equipment of forthcoming this epidemic of soreness indicates nice promise. In Analgesia: tools and Protocols, specialists within the box current thorough insurance of molecular analgesia study equipment from aim discovery via goal validation and scientific checking out to tolerance and dependence, with broad chapters on rising receptor periods as objectives for analgesic medicines and leading edge analgesic ideas. As a quantity within the hugely profitable Methods in Molecular Biology™ sequence, the chapters comprise introductions to their respective themes, lists of the mandatory fabrics and reagents, step by step, with ease reproducible protocols, and notes sections with pointers on troubleshooting and fending off recognized pitfalls.
Comprehensive and crucial, Analgesia: equipment and Protocols can provide to assist and improve the learn of all these scientists and clinicians who're attracted to what the more and more molecular destiny has in shop for analgesia examine, from the molecular examine bench during the animal laboratory to the bedside.
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Extra resources for Analgesia: Methods and Protocols
Adult male Sprague-Dawley rats weighing 250–350 g are housed (lights on from 7:00 am to 7:00 pm) with water and food ad libitum. Pentobarbital. 2. Surgical Tools 1. Scissors, straight and curved, Forceps, Mosquito, Hemostat, Retractor, Clipper, Staples, Needle and needle holder (FST, Heidelberg, Germany). Blade holder and surgical blade 22/11. Suture, Silk, Nylon, and Chromic gut. High intensity illuminator; Fiber-Lite MI-150 (Dolan-Jenner, Andover, MA). 3. The standard Semmes–Weinstein set of von Frey hairs (Stoelting, Wood Dale, IL).
Pain 22:1–31 47. Stanley KL, Paice JA (1997) Animal models in pain research. Semin Oncol Nurs 13:3–9 Chapter 4 Animal Models of Acute and Chronic Inflammatory and Nociceptive Pain Janel M. Boyce-Rustay, Prisca Honore, and Michael F. Jarvis Abstract To facilitate the study of pain transmission and the characterization of novel analgesic compounds, an array of experimental animal pain models has been developed mainly in rodents. In these preclinical models, nociceptive pain can be measured by both spontaneous and evoked behaviors.
Recently, several animal models have been developed to investigate the mechanism of pain and provide new information on the treatment of pain. The pathophysiology of acute pain is now better understood owing to the development of such animal models. Each of these animal models may mimic a specific clinical condition of acute surgical pain. This chapter summarizes the animal models of acute surgical pain currently used in the pain research field. 2. 1. Experimental animals 1. Adult male Sprague-Dawley rats weighing 250–350 g are housed (lights on from 7:00 am to 7:00 pm) with water and food ad libitum.