By Norman H. Crowhurst
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2 Note: All assays using acetone and MEK solvents in ITLC were per- formed on the same sample vials. 5. 5, the ITLC procedures used for these materials are accurate to within 2% of the actual value of 99mTcOi present. 6. 7 for 99mTc-DisIDA and 99mTc-Glucoheptonate. 1) is accurate to within -2% of the true value. 3. 99mTcOi Measurements by lTLC of 99mTc-Glucoheptonate with Development of Wet vs. 4% 99mTcOi added to* 99mTc-Glucoheptonate after addition of excess WTcOi 33% 99mTcOi added to* 99mTc-Glucoheptonate after addition of excess 99TcOi a Duplicate assays of same vial.
Chromatogr. HPLC Magzn. 1:282-289, 1983. F. : Contemporary Practice of Chromatography. Elsevier, Amsterdam, 1984, pp. 619-700. , Piet, L. : Some quantitative aspects of high performance thin-layer chromatography. 1. Liquid. , 1985, in press. 36 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. Instrumental Evaluation of Thin-Layer Chromatograms Halpaap, H. : Thin-layer chromatographic and high-performance thin-layer chromatographic ready-for-use preparations with concentrating zones.
Quantitative measurements can be made with a photodensitometer to measure the darkening of the developed film. The disadvantages of autoradiography are that the film may require extremely long exposure times, it is difficult to quantify, particularly for wide ranges of radioactivity, and it can show false images as a result of chemical interactions between the TLC layer and the film. , 3H), a large part of their energy is absorbed by the chromatographic layer so that only a few electrons reach the photographic emulsion.