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Not only are the optical spectra of these components extremely difficult to resolve, but also EPR techniques applied at temperatures above that of liquid nitrogen (77°K) detect only a small fraction of the iron sulfur centers in the mitochondrial membrane. Since EPR techniques are not commonly used in the field of yeast biochemistry, the principles of this technique will be first described in comparison with the spectrophotometry. Electron paramagnetic resonance (EPR) measures absorption of microwave energy by unpaired electrons in the sample in an l EPR¿ electron paramagnetic resonance; ETF3 electron transferring flavoprotein; SDH3 succinate dehydrogenase; SMP¿ submitochondrial particles; UQ> ubiquinone; USQ¿ ubisemiquinone.

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