By Angela Dillow, Anthony Lowman

Offers easy methods to advance man made and organic fabrics with more desirable in vivo homes. Considers the influence of topographical gains on cellphone behaviors equivalent to orientation, adhesion, migration, proliferation, and differentiation.

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TM Winger, PJ Ludovice, EL Chaikof. Lipopeptide conjugates: biomolecular building blocks for receptor activating membrane-mimetic structures. Biomaterials 17(4):437–441, 1996. 50. N Oku, C Koike, Y Tokudome, S Okada, N Nishidawa, H Tsukada, M Kiso, A Hasegawa, H Fujii, J Murata, I Saiki. Application of liposomes for cancer metastasis. Adv Drug Del Rev 24(2-3):215–223, 1997. 51. B Hu, D Finsinger, K Peter, Z Guttenberg, M Barmann, H Kessler, A Escherich, L Moroder, J Bohm W Baumeister, SF Sui, E Sackmann.

1) (19,52). The applied shear stress t (force/area) at any point on the surface of the disk varies linearly with radial position and is given by: pffiffiffiffiffiffiffiffiffiffiffi t ¼ 0:800 r rmo3 ð1Þ where r is radial position from the disk center, r and m are fluid density and viscosity, and o is angular speed. Following uniform cell seeding and spinning, cells are fixed, stained, and counted at specific radial positions. The fraction of adherent cells (cell counts normalized to counts at the center of the disk [no applied force]) decreases nonlinearly with shear stress (Fig.

Extensive control experiments have indicated that this method is specific for ligated integrins. © 2002 by Marcel Dekker, Inc. We have used this methodology to quantify initial as well as long-term integrin binding to FN (48,51). For quantification of initial integrin binding, human fibroblasts were plated on different FN surface densities for 15 min and bound integrins were cross-linked and recovered. The amount of recovered a5 (Fig. 4) and b1 integrin subunits increases with FN surface density, indicating increases in bound a5 b1 integrin as a function of FN surface density.

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