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Professor; Cell Biology and Neuroanatomy; University of Minnesota Twin Cities 200 Oak Street Se Minneapolis, Mn 554552070 Timing: Fiscal Year 2001; Project Start 01-SEP-1985; Project End 31-MAR-2002 Summary: A growth cone is the motile tip of a growing axon or dendrite. The proper development of neural circuits depends on the pathfinding activities of growth cones. Five behaviors of growth cones are critical in the formation of neural circuits: growth cone migration, turning, branching, retraction and synaptogenesis.

Some existing 50 chondrocytic strains are available for study. The consequence(s) of constitutive expression of human telomerase reverse transcriptase (hTERT) will be determined. The human cDNA clone for hTERT has been cloned in the retroviral expression system pLXSN for ready transfection to selected human cell strains. Introduction of this gene has been shown to immortalize cells of some systems. Reports with chondrocyte cell lines are needed. Functionality and doubling potential of chondrocytic lines will be assessed both before and after transfection.

Associate Professor; University of California San Diego 9500 Gilman Dr, Dept. 0934 La Jolla, Ca 92093 Timing: Fiscal Year 2003; Project Start 01-FEB-2003; Project End 31-JAN-2008 Summary: This project proposes to investigate the functions of dermatan sulfate in vascular biology and inflammation. Dermatan sulfate is the major glycosaminoglycan expressed in the extracellular matrix of the skin and has recently been found to be expressed on the luminal cell surface of vascular endothlium. Dermatan sulfate shares many functions with heparan sulfate but has been much less well studied.

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