By George G. Rose
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Additional info for Cinemicrography in Cell Biology
A g a i n the striking similarity between strains 929 and 3500 was apparent. O f 2 2 0 cells o f strain 2 0 7 1 , w h i c h together with their d a u g h t e r cells were completely observable for the e n t i r e d u r a t i o n o f o n e 53-hour a n d o n e 57-hour film sequence, only 7 % failed to show any division at all (Fig. 12). Seventy-one per cent showed only the p a r e n t cell dividing with n e i t h e r daughter cell dividing. E l e v e n per cent o f the cells showed 0 10 20 GENERATION 30 40 50 60 TIME OF ONE CELL OF SISTER PAIR (hours) FIG.
STRAIN 929 (32 CELLS) PERCENT FIG. 11. T h e percentage of cells observed to divide, which further showed: (a) neither daughter cell, (b) one daughter cell, or (c) both daughter cells dividing during 53- and 57-hour film sequences in strains N C T C 929, 2071, and 3500. 47 CINEMICROGRAPHIC E Q U I P M E N T C o m p a r i s o n o f the t h r e e cell strains w i t h respect to t h e of cells dividing, in w h i c h (a) n e i t h e r d a u g h t e r proportion cell, (b) o n e daughter cell, o r (c) b o t h d a u g h t e r cells divided d u r i n g film sequences o f 53 a n d 57 h o u r s is shown in F i g .
All studies h a d to b e m a d e using the simultaneous b e h a v i o r o f the original cells as a c o n t r o l . T h i s neces sitated simultaneous c i n e m i c r o g r a p h i c records o f b o t h n o r m a l a n d treated cultures. T h e r e f o r e , a comparison o r d o u b l e c i n e m i c r o g r a p h i c c a m e r a was constructed a n d the description o f this earlier i n s t r u m e n t has already b e e n published ( E a r l e a n d Crisp, 1943). C e r t a i n inadequacies developed in the use of this i n s t r u m e n t so that at a later date this e q u i p m e n t was r e b u i l t to give it greater ver satility.